MEK2 (MAP2K2) Antibody (N-term)

SKU: orb1928730

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD5717
• Immunogen: This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MEK2 (MAP2K2). Antigen Region: 1-30 aa.
• Target: MAP2K2
• Molecular Weight: 44424 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2, MEK2, MKK2, PRKMK2

MEK2 (MAP2K2) Antibody (N-term) western blot analysis in Hela, MCF-7, rat C6 cell line and mouse Liver, rat Brain tissue lysates (35 ug/lane).This demonstrates the MEK2 (MAP2K2) antibody detected the MEK2 (MAP2K2) protein (arrow).

All lanes: Anti-MEK2 (MAP2K2) Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of U251 cell stained with MEK2 (MAP2K2) Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 (MAP2K2) primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MEK2 (MAP2K2) immunoreactivity is localized to Cytoplasm significantly.

Quick Database Links

Gene Symbol

MAP2K2

UniProt

P36507

RefSeq (Protein):NP_109587.1