MAP2K1 Antibody

SKU: orb1239447

Description

MAP2K1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Gallus, Rabbit
• Application Notes:
  MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MEK1/2 antibody was raised against a 15 amino acid peptide near the center of human MEK1.The immunogen is located within amino acids 240 - 290 of MEK1/2.
• Target: MAP2K1
• Molecular Weight: Predicted: 43 kDa Observed: 44 kDa
• Purification: MEK1/2 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: MEK1/2 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mitogen-activated protein kinase kinase 1, MAP2K1, MAPKK1, MEK1, MKK1, PRKMK1, CFC3, Mitogen-activated protein kinase kinase 2, MAP2K2, MAPKK2, MEK2, MKK2, PRKMK2, CFC

Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 5 µg/mL.

Immunofluorescence of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

MAP2K1

UniProt

Q0VD16

RefSeq (Protein):NP_002746