MEK1 (MAP2K1) Antibody (N-term)

SKU: orb1928734

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This MEK1 (MAP2K1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MEK1 (MAP2K1). Antigen Region: 1-30 aa.
• Target: MAP2K1 (HGNC:6840)
• Molecular Weight: 43439 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1, MEK1, PRKMK1

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of lysates from Jurkat, rat PC-12, mouse NIH/3T3, rat C6 cell line and mouse brain tissue (from left to right), using MAP2K1 Antibody (P1).Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Quick Database Links

Gene Symbol

MAP2K1 (HGNC:6840)

UniProt

Q02750

RefSeq (Protein):NP_002746.1