MAP2K1 Antibody

SKU: orb420334

Description

MEK1 antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:40,000, IHC: 1:100, WB: 1µg/mL
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-MEK 1 (RABBIT) antibody is tested in ELISA, Western Blotting, and IHC. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
• Target: MAP2K1
• Purity: This affinity purified antibody is directed against human MEK1 protein. Anti-MEK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-MEK1 antibody, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MAP2K1, MEK, MEK 1, MKK1, PRKMK1, MEK-1, ERK activator kinase 1, MAPK/ERK kinase 1

Quick Database Links

Gene Symbol

MAP2K1

UniProt

Q02750