MEK1 C-Term Antibody

SKU: orb420333

Description

MEK1 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:40,000, IHC: 1:100, WB: 1µg/mL
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-MEK 1 (RABBIT) antibody is tested in ELISA, Western Blotting, and IHC. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
• Purity: This affinity purified antibody is directed against human MEK1 protein. Anti-MEK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-MEK1 antibody, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MAP2K1, MEK, MEK 1, MKK1, PRKMK1, MEK-1, ERK activator kinase 1, MAPK/ERK kinase 1

Immunohistochemistry with anti-MEK1 (C-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK1 (C-Term) antibody orb420333 at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.

Western Blot of Anti-MEK1 Antibody. Lane 1: Molecular Weight Marker. Lane 2: MEK1 recombinant lysate. Lane 3: MEK2 recombinant lysate. Load: 10 µg. Primary Antibody: Anti-MEK1 (c-term) at 1 µg/ml overnight at 4°C. Secondary Antibody: Goat anti-Rabbit peroxidase conjugated antibody at 1:40000 for 30 minutes RT. Blocking: BlockOut Universal Blocking buffer (p/n orb348644). Predicted Size: 43.5kDa.

Western Blot of Rabbit anti-MEK1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: HeLa Lysate (p/n orb348668). Lane 3: MCF-7 Lysate (p/n orb348664). Lane 4: Jurkat Lysate. Lane 5: A431 Lysate (p/n orb348665). Lane 6: A549 Lysate (p/n orb348675). Lane 7: LNCap Lysate (p/n orb348694). Lane 8: MOLT-4 Lysate (p/n orb348696). Lane 9: Ramos Lysate. Lane 10: Raji Lysate (p/n orb348672). Lane 11: A-172 Lysate (p/n orb348708). Lane 12: NIH/3T3 Lysate (p/n orb348714). Load: 35 µg per lane. Primary antibody: MEK1 antibody at 1 ug/ml overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n orb348624) for 30 min at RT. Predicted/Observed size: 43kDa for MEK1.

Quick Database Links

UniProt

Q02750