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MEK1 C-Term Antibody FITC
Catalog Number: orb420450
| Catalog Number | orb420450 |
|---|---|
| Category | Antibodies |
| Description | MEK1 antibody (FITC) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | FITC |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Lyophilized |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | This fluorescein conjugated affinity purified antibody is directed against human MEK1 protein. Anti-MEK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform. |
| Immunogen | Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH. |
| UniProt ID | Q02750 |
| Tested applications | FLISA, IHC, WB |
| Dilution range | FLISA: 1:40,000, IHC: 1:100, WB: 1µg/ml |
| Application notes | Anti-MEK 1 Fluorescein conjugated antibody has been tested by western blot and is suitable for use in ELISA. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa. |
| Antibody Type | Primary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | rabbit anti-MEK1 antibody fluorescein conjugation, Read more... |
| Note | For research use only |
Western Blot of Rabbit Anti-MEK1 (C-term) FITC Conjugated Antibody. Lane 1: Opal Prestained Molecular Weight Marker (5 µl). Lane 2: MEK1 human recombinant protein (p/n orb420175) (0.1 µg). Primary Antibody: Anti-MEK1 FITC conjugated at 1 µg/ml overnight at 2-8°C. Secondary Antibody: none. Block: BlockOut Buffer (p/n orb348644). Expected MW: ~47kDa.
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