MAP2K1 Antibody (Biotin)

SKU: orb420266

Description

MEK1 antibody (Biotin)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:40,000, WB: 1µg/mL
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-MEK 1 Biotin Conjugated (MOUSE) Antibody has been tested by Western Blotting and ELISA. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 13B6.G12
• Immunogen: Anti-MEK1 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
• Target: MAP2K1
• Purity: This biotin conjugated protein A purified mouse monoclonal antibody reacts specifically with human MEK1. Anti-MEK1 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

mouse anti-MEK1 antibody biotin conjugation, biotin conjugated mouse anti-MEK1 antibody, MAP2K, MEK, MEK 1, MKK1, PRKMK1, MEK-1 Antibody

Western Blot of Anti-MEK1 Antibody. Lane 1: MEK-1 recombinant protein. Lane 2: MEK-2 recombinant protein. Load: 50 ng per lane. Primary Antibody: Anti-MEK1 supernatant clone neat over night at 4°C. Secondary Antibody: Anti-mouse HRP at 1:40000 dilution.

Quick Database Links

Gene Symbol

MAP2K1

UniProt

Q02750