MEK-1 (phospho Thr292) rabbit pAb Antibody

SKU: orb769695

Description

Rabbit polyclonal antibody to MEK-1 (phospho-Thr292)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human MEK1 around the phosphorylation site of Thr291. AA range:261-310
• Molecular Weight: 43
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-MAP2K1 antibody, anti-MEK1 antibody, anti-PRKMK1 antibody, anti-Dual specificity mitogen-activated protein kinase kinase 1 antibody, anti-MAP kinase kinase 1 antibody, anti-MAPKK 1 antibody, anti-MKK1 antibody, anti-ERK activator kinase 1 antibody, anti-MAPK/ERK kinase 1 antibody, anti-MEK 1 antibody

Western Blot analysis of 293 cells using Phospho-MEK-1 (T292) Polyclonal Antibody diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MEK1 (Phospho-Thr291) Antibody.

Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr291) Antibody. The lane on the right is blocked with the phospho peptide.