MED21 Antibody / SRB7

SKU: orb2636954

Description

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors. Med6 and Med7, as well as other components of SMCC, associate with co-activator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation. Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity

Images & Validation

• Tested Applications: ELISA, FACS, IF, WB
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the MED21 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: PCRP-MED21-4B5
• Immunogen: Recombinant full-length human MED21 protein was used as the immunogen for the MED21 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

FACS staining of PFA-fixed human U-87 cells with MED21 antibody (blue, clone PCRP-MED21-4B5), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free MED21 antibody (clone PCRP-MED21-4B5) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human U-87 cells using MED21 antibody (green, clone PCRP-MED21-4B5) and phalloidin (red).

Immunofluorescent staining of PFA-fixed human HeLa cells using MED21 antibody (green, clone PCRP-MED21-4B5) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with MED21 antibody (blue, clone PCRP-MED21-4B5), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using MED21 antibody (clone PCRP-MED21-4B5). These results demonstrate the foremost specificity of the PCRP-MED21-4B5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

Q13503