MED14 Antibody (Center)

• Catalog Number: orb1937663
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB20179
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:4000, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 160607 Da
• Target: This MED14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-615 amino acids from the Central region of human MED14.
• UniProt ID: O60244
• NCBI: NP_004220.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mediator of RNA polymerase II transcription subuni
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-MED14 Antibody (Center) at 1:4000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: DU145 whole cell lysate. Lane 4: SW480 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 160 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

MED14 Antibody (Center) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the MED14 antibody detected the MED14 protein (arrow).

Overlay histogram showing A549 cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.