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Item 1 of 4
Item 1 of 4
Me2-H4(K20) Antibody
Catalog Number: orb1931072
| Catalog Number | orb1931072 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB17115 |
| Tested applications | DOT, FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50, DB: 1:500 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 11367 Da |
| Target | This H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human H4. |
| UniProt ID | P62805 |
| NCBI | NP_003532.1, NP_068803.1, NP_003539.1, NP_001029249.1, NP_003529.1, NP_003533.1, NP_003536.1, NP_003535.1, NP_003534.1, NP_003486.1, NP_778224.1, NP_003531.1, NP_003537.1, NP_003530.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Histone H4, HIST1H4A, H4/A, H4FA Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
H4 Antibody (K20 [Me2]) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Dot blot analysis of anti-hH4-K20 (Methyl 2) methylation-specific Pab on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Western blot analysis of lysate from K562 cell line, using H4 Antibody (K20). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.
