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MDM2 Antibody
SKU: orb535748
Description
Research Area
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB (1:1000); IHC (1:50) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide from the mid-protein of Human MDM2 (aa. 100-200) |
| Target | MDM2 |
| Molecular Weight | 50 kDa |
| Purification | Peptide Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−MDM2, Double minute 2 protein, Hdm2, MTBP, Mouse Double Minute 2, HDMX, ACTFS, p53 Binding Protein Mdm2, Ubiquitin protein ligase E3 Mdm2, Mdm2 p53 E3 ubiquitin protein ligase homolog, MDM2BP, p53-binding protein Mdm2, Oncoprotein Mdm2, Hdm 2, E3 ubiquitin-protein ligase Mdm2, MDM 2, EC:6.3.2.-, MDM2_HUMAN, MDM2 oncogene E3 ubiquitin protein ligase, Murine Double Minute Chromosome 2, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa
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Immunohistochemistry analysis using Rabbit Anti-MDM2 Polyclonal Antibody. Tissue: Stomach Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western blot analysis of Mouse, Rat Brain showing detection of ~40 kDa MDM2 protein using Rabbit Anti-MDM2 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~40 kDa. Other Band (s): Isoform 2 or 3.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001138811.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
MDM2 Antibody (orb535748)
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