Mdm2 Antibody

SKU: orb345546

Description

MDM2 antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:3,000 - 1:12,000, IP: 1:100, WB: 1:500 - 1:2,000
• Reactivity: Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to MDM2 protein by western blotting in the appropriate cell lysate or extract. See Saucedo, et al (1999) for a discussion on expected molecular weights.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 175-200 of mouse MDM2.
• Target: Mdm2
• Purity: This affinity-purified antibody is directed against mouse MDM2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against Mouse MDM2 protein. A BLAST analysis was used to suggest minimal cross reactivity with MDM2 homologues from other sources.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-MDM2 antibody, MDM-2, E3 ubiquitin-protein ligase Mdm2, p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, RING-type E3 ubiquitin transferase Mdm2, Double minute 2 protein, Hdm2

Western blot using Biorbyt's affinity purified Anti-MDM2 (Rabbit) is shown to detect a band (arrow) corresponding to mouse MDM2 protein. Lane 1: human kidney HEK293 cells (p/n orb348669). Lane 2: mouse MEF cells (p/n orb348722). Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% normal goat serum, 0.5% BLOTTO (p/n orb348624) in PBS, the membrane was probed for overnight at 4° with the primary antibody diluted to 1:500 in 1% normal goat serum, 0.1% BLOTTO in PBS. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).

Quick Database Links

Gene Symbol

Mdm2

UniProt

P23804

RefSeq:2851543