MDM2 Antibody: Biotin

• Catalog Number: orb535759
• Category: Antibodies
• Description: Rabbit polyclonal antibody against MDM2 conjugated to Biotin
• Target: MDM2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Biotin
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Purification: Peptide Affinity Purified
• Immunogen: Synthetic peptide from the mid-protein of Human MDM2 (aa. 100-200)
• UniProt ID: Q00987
• MW: 50 kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); IHC (1:50)
• Application notes: #REF!
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Double minute 2 protein antibody, Hdm2 antibody, M
• Research Area: Cancer Biology, Cell Biology, Epigenetics & Chroma
• Note: For research use only
• Entrez: 4193
• NCBI: NP_001138811.1

Immunohistochemistry analysis using Rabbit Anti-MDM2 Polyclonal Antibody. Tissue: Stomach Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse, Rat Brain showing detection of ~40 kDa MDM2 protein using Rabbit Anti-MDM2 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~40 kDa. Other Band (s): Isoform 2 or 3.