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MDK Antibody (C-term)
Catalog Number: orb1937747
| Catalog Number | orb1937747 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB14452 |
| Tested applications | IF, IHC-P, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:2000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 15585 Da |
| Target | This MDK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the C-terminal region of human MDK. |
| UniProt ID | P21741 |
| NCBI | NP_002382.1, NP_001257479.1, NP_001012333.1, NP_001012334.1, NP_001257480.1, NP_001257481.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Midkine, MK, Amphiregulin-associated protein, ARAP Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-MDK Antibody (C-term) at 1:2000 dilution + Human pancreas lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 16 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of MDK Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
MDK Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MDK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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