MDFIC Antibody (N-term)

• Catalog Number: orb39401
• Category: Antibodies
• Description: Peptide affinity purified rabbit polyclonal antibody.
• Target: This MDFIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-99 amino acids from the N-terminal region of human MDFIC.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: Synthetic Peptide
• UniProt ID: Q9P1T7
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Antibody Type: Primary Antibody
• Clone Number: RB22782
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-MyoD family inhibitor domain-containing prote
• Research Area: Cell Biology, Microbiology, Signal Transduction
• Note: For research use only
• NCBI: NP_951038.1, NP_001159817.1

Western blot analysis of MDFIC Antibody (N-term) in Hela cell line lysates (35 ug/lane). MDFIC (arrow) was detected using the purified Pab.

MDFIC Antibody (N-term) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human spleen reacted with MDFIC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.