MDC1 Antibody: APC

• Catalog Number: orb148041
• Category: Antibodies
• Description: Mouse monoclonal to MDC1 (APC). MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A. X on S139..
• Target: MDC1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: APC
• Reactivity: Bovine, Human, Mouse, Primate
• Concentration: 1 mg/ml
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Purification: Protein G Purified
• Immunogen: GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
• UniProt ID: Q5PSV9
• MW: 184kDa
• Tested applications: ICC, IF, IHC
• Dilution range: WB (1:2000), ICC/IF (1:100)
• Application notes: 0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
• Clone Number: P2B11
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Nuclear factor with BRCT domains1 antibody, mediat
• Research Area: Cancer Biology, Cell Biology, Epigenetics & Chroma
• Note: For research use only
• Entrez: 240087
• NCBI: NP_001010833.2

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.

Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.