MDC Antibody

• Catalog Number: orb1272767
• Category: Antibodies
• Description: MDC Antibody
• Target: Ccl22
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMDC. Murine MDC specific antibody was purified by affinity chromatography employing immobilized mMDC matrix.
• UniProt ID: O88430
• Tested applications: ELISA, NeA, WB
• Application notes: Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mMDC (100 ng/mL), a concentration of 6-9 μg/mL of this antibody is required. ELISA:Indirect:To detect mMDC by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC.SandwichTo detect mMDC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MDC as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC. Western Blot:To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MDC, DCBCK, ABCD-1, Scya22, Abcd1, C-C motif chemo
• Note: For research use only
• NCBI: O88430

To detect mMDC by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MDC (orb1272766) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC.

To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.