Ccl22 Antibody

SKU: orb1272767

Description

Ccl22 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Mouse
• Application Notes:
  Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mMDC (100 ng/mL), a concentration of 6-9 μg/mL of this antibody is required. ELISA:Indirect:To detect mMDC by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC.SandwichTo detect mMDC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MDC as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC. Western Blot:To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMDC. Murine MDC specific antibody was purified by affinity chromatography employing immobilized mMDC matrix.
• Target: Ccl22
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MDC, DCBCK, ABCD-1, Scya22, Abcd1, C-C motif chemokine 22, Activated B and dendritic cell-derived,

To detect mMDC by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MDC (orb1272766) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC.

To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

Ccl22

UniProt

O88430

RefSeq:O88430