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Catalog Number | orb1272395 |
---|---|
Category | Antibodies |
Description | MCP-3 Antibody (biotin) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMCP-3 (human MCP-3). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Biotin |
Target | CCL7 |
UniProt ID | P80098 |
NCBI | P80098 |
Storage | MCP-3 antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles. |
Alternative names | FIC, MARC, MCP3, NC28, MCP-3, SCYA6, SCYA7, C-C mo Read more... |
Note | For research use only |
Application notes | ELISA:Sandwich:To detect hMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human MCP-3 (XP-5222) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-3.Western Blot:To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect hMCP-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human MCP-3 (orb1272396) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMCP-3.
To detect by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
IHC, WB | |
Equine, Human, Porcine | |
Rabbit | |
Polyclonal | |
Biotin |
IF, IHC-Fr, IHC-P | |
Mouse | |
Rat | |
Rabbit | |
Polyclonal | |
Biotin |