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MCP-1/MCAF Antibody

Catalog Number: orb1272404

DispatchUsually dispatched within 5-10 working days
$ 740.00
Catalog Numberorb1272404
CategoryAntibodies
DescriptionMCP-1/MCAF Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman
ImmunogenProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP 1/MCAF (human Macrophage/Monocyte chemotactic protein-1).
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLyophilized
ConjugationUnconjugated
TargetCCL2
UniProt IDP13500
NCBIP13500
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HS
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NoteFor research use only
Expiration Date12 months from date of receipt.
MCP-1/MCAF Antibody

To detect hMCP-1 (MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human MCP-1 (MCAF) (orb1272403) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1 (MCAF).

MCP-1/MCAF Antibody

To detect hMCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 (MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

MCP-1/MCAF Antibody

To detect hMCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 (MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

MCP-1/MCAF Antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 ug/ml and a two-hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

MCP-1/MCAF Antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 ug/ml and a two-hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

MCP-1/MCAF Antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 ug/ml and a two-hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

  • MCP-1/MCAF Antibody [orb1272402]

    ELISA,  NeA,  WB

    Rat

    Rabbit

    Polyclonal

    Unconjugated

    0.1 mg
  • MCP-1/MCAF Antibody (biotin) [orb1272401]

    ELISA,  WB

    Rat

    Rabbit

    Polyclonal

    Biotin

    0.05 mg
  • MCP-1 / MCAF antibody [PD-5] [orb1973108]

    IF,  IHC,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μl, 25 μl
  • MCP-1/MCAF Antibody (biotin) [orb1272403]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    Biotin

    0.05 mg