Human Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit

SKU: orb779110

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1(MCP1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MCP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.4 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CCL2 ELISA Kit, GDCF2 ELISA Kit, HC11 ELISA Kit, HSMCR30 ELISA Kit, MCAF ELISA Kit, MCP1 ELISA Kit, SCYA2 ELISA Kit, SMC-CF ELISA Kit, Chemokine C-C-Motif Ligand 2 ELISA Kit, Monocyte Chemotactic And Activating Factor ELISA Kit, Monocyte Secretory Protein JE ELISA Kit

Quick Database Links

UniProt

P13500

References

Kamila Pasternak-Mnich 1, Jolanta Kujawa 2, Justyna Agier 3, Elżbieta Kozłowska 3 Impact of photobiomodulation therapy on pro-inflammation functionality of human peripheral blood mononuclear cells - a preliminary study Sci Rep ., (2024)

PubMed: 39367102

Applications

ELISA

Reactivity

Human