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MCP-1/MCAF Antibody (biotin)
Catalog Number: orb1272401
| Catalog Number | orb1272401 |
|---|---|
| Category | Antibodies |
| Description | MCP-1/MCAF Antibody (biotin) |
| Target | Ccl2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Biotin |
| Reactivity | Rat |
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat MCP-1(MCAF) (rat MCP-1(MCAF)). |
| UniProt ID | P14844 |
| Tested applications | ELISA, WB |
| Application notes | ELISA:Sandwich:To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat MCP-1(MCAF) (XP-5219) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF). Western Blot:To detect rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat MCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Antibody Type | Primary Antibody |
| Storage | MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles. |
| Alternative names | MCP-1, Scya2, Sigje, Je, Mcp1, C-C motif chemokine Read more... |
| Note | For research use only |
| NCBI | P14844 |
To detect Rat MCP-1 (MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Rat MCP-1 (MCAF) (orb1272402) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat MCP-1 (MCAF).
To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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