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Catalog Number | orb1257980 |
---|---|
Category | Antibodies |
Description | MCM4 Antibody |
Target | MCM4 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | A synthetic peptide of human MCM4 |
UniProt ID | P33991 |
MW | Observed: 105kDa |
Tested applications | IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | MCM4, NKCD, CDC21, CDC54, NKGCD, hCdc21, P1-CDC21 |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using MCM4 antibody (orb1257980) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat pancreas using MCM4 Antibody (orb1257980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using MCM4 Antibody (orb1257980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using MCM4 Antibody (orb1257980) at dilution of 1:100 (40x lens).
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |