MCM2 (2B5) Mouse mAb

SKU: orb1563145

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Description

Research Area

Epigenetics & Chromatin

Images & Validation

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Item 1 of 6

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, IP, WB
Dilution Range	WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
Reactivity	Human, Mouse

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	IgG1
Clone No.	2B5-H4-A9
Immunogen	Purified recombinant fragment of human MCM2 expressed in E. Coli.
Target	MCM2
Molecular Weight	Calculated MW: 102 kDa; Observed MW: 125 kDa
Purification	Ascitic Fluid
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

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Western blot analysis of MCM2 in Jurkat, K562, 3T3 and Hela lysates using MCM2 antibody.

Immunoprecipitation analysis of MCM2 (2B5) in Hela lysates using MCM2 antibody.

Immunofluorescence analysis of MCM2 (2B5) in Hela cells using MCM2 antibody (green), and DAPI (blue).

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MCM2 (2B5) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry analysis of paraffin-embedded ovarian cancer tissues using MCM2 (2B5) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.