Mcl-1 (phospho Ser159) rabbit pAb

• Catalog Number: orb769080
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Mcl-1 (phospho-Ser159)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human MCL1 around the phosphorylation site of Ser159. AA range:125-174
• MW: 39
• Tested applications: ELISA, IF, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MCL1 antibody, anti-BCL2L3 antibody, anti-Ind
• Note: For research use only

Western blot analysis of K562 using p-Mcl-1 (S159) antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MCL1 (Phospho-Ser159) Antibody.

Immunofluorescence analysis of A549 cells, using MCL1 (Phospho-Ser159) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).