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| Catalog Number | orb1269519 |
|---|---|
| Category | Antibodies |
| Description | MCF2 Antibody |
| Target | MCF2 |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Immunogen | This MCF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 804-833 amino acids from the C-terminal region of human MCF2. |
| UniProt ID | P10911 |
| MW | 108 kDa |
| Tested applications | FC, IHC-P, WB |
| Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Proto-oncogene DBL, Proto-oncogene MCF-2, MCF2-tra Read more... |
| Research Area | Cancer Biology, Signal Transduction |
| Note | For research use only |
| NCBI | P10911 |

Western blot analysis in K562 cell line lysates (35 ug/lane).

MCF2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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