MC4 Receptor Polyclonal Antibody

• Catalog Number: orb1421140
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MC4 Receptor.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Rat
• UniProt ID: P32245
• Tested applications: ELISA, ICC, IF, IHC-Fr, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Melanocortin receptor 4, MC4-R, MC4R
• Note: For research use only

Sample: Embryo (Mouse) Lysate at 40 ug. Primary: Anti- MC4 Receptor at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 37 kD. Observed band size: 52 kD.

Sample: MCF-7 Cell (Human) Lysate at 40 ug. Primary: Anti-MC4 Receptor at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 37 kD. Observed band size: 40 kD.

Positive control: RSC96. Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1: 100 in 1 X PBS containing 0.5% BSA; Primary Antibody Dilution: 6 µg in 100 µL 1X PBS containing 0.5% BSA.

Blank control: 293T (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).Primary Antibody: Rabbit Anti-MC4 Receptor /AF488 Conjugated antibody, Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.

Blank control: 293T (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-MC4 Receptor /AF488 Conjugated antibody, Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.