MBP Antibody / Myelin Basic Protein

• Catalog Number: orb699746
• Category: Antibodies
• Description: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. Phosphorylation been shown to decrease its ability to aggregate lipid vesicles and consequently destabilising the compact structure ofmyelin, a destruction that has been observed in demyelinating diseases such as Multiple Sclerosis. MBP/4276 is a useful detector of phosphorylated MBP by binding to Thr98 of human MBP in the phosphorylated state.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A synthetic peptide corresponding to the amino acids surrounding phosphorylated threonine 98 were used as the immunogen for the Myelin Basic Protein antibody.
• UniProt ID: P02686
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: MBP/4276
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Myelin Basic Protein antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4276). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4276). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human brain lysate with Myelin Basic Protein antibody (clone MBP/4276). Isoforms may be visualized from 20~37 kDa.

SDS-PAGE analysis of purified, BSA-free Myelin Basic Protein antibody (clone MBP/4276) as confirmation of integrity and purity.