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MBP Antibody / Myelin Basic Protein

Catalog Number: orb699743

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb699743
CategoryAntibodies
DescriptionMyelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4273 recognizes an epitope in the 82-87 region of MBP, useful in clinical diagnosis to detect MBP levels or myelination in human MBP. MBP/4273 is also used in a 2-site ELISA with clone MBP/4275. Both are used interchangeably as capture and detection antibody.
Species/HostRat
ClonalityMonoclonal
Clone NumberMBP/4273
Tested applicationsIHC-P, WB
ReactivityHuman, Mouse
IsotypeRat IgG2a
ImmunogenThe epitope for this Myelin Basic Protein antibody has been mapped to the 82-87 amino acid region (DENPVV).
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis Myelin Basic Protein antibody is available for research use only.
UniProt IDP02686
StorageStore the Myelin Basic Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
MBP Antibody / Myelin Basic Protein

IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4273). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

MBP Antibody / Myelin Basic Protein

IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4273). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

MBP Antibody / Myelin Basic Protein

Western blot testing of human brain lysate with Myelin Basic Protein antibody (clone MBP/4273). Isoforms may be visualized from 20~37 kDa.

MBP Antibody / Myelin Basic Protein

SDS-PAGE analysis of purified, BSA-free Myelin Basic Protein antibody (clone MBP/4273) as confirmation of integrity and purity.

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    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
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    IHC-P

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg
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    IHC-P,  WB

    Human

    Mouse

    Recombinant

    Unconjugated

    20 μg
  • MBP Antibody / Myelin Basic Protein [orb699741]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg