MBP Antibody / Myelin Basic Protein

• Catalog Number: orb2636398
• Category: Antibodies
• Description: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. Clone rMBP/9301 is used in a two-site ELISA with clone MBP/4273 (cat # V8677). Both are used interchangeably as capture and detection antibody.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment (within amino acids 1-157) of human Myelin Basic Protein was used as the immunogen for the recombinant MBP antibody.
• UniProt ID: P02686
• Tested applications: ELISA, IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,ELISA: pairs with cat # V8677
• Application notes: Optimal dilution of the recombinant MBP antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rMBP/9301
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant MBP antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human brain tissue with recombinant MBP antibody (clone rMBP/9301). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified recombinant MBP antibody (clone rMBP/9301) as confirmation of integrity and purity.