MBL-A, Mouse, mAb 8G6 Antibody

SKU: orb1109172

Description

Mouse monoclonal antibody to MBL-A

Images & Validation

• Tested Applications: IA, IF, IHC-Fr, WB
• Dilution Range: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Reactivity: Mouse
• Application Notes:
  W: A non-reduced sample treatment was used. The band sizes are 191, 263 and 316 kDa (Ref.1). IHC-F: Tissue sections were fixed in 4% PBS-buffered formaldehyde and pretreated with 2% hydrogen peroxide in methanol for 20 minutes at 4°C to quench endogenous peroxidases. Primary antibody 8g6, 2µg/ml. As negative control rat IgG2a was used (Ref.2). MBL-A (8G6) deposition in developing murine atherosclerotic lesions. Staining of frozen tissue sections with antibody 8G6. Anti-mouse MBL-A at 2µg/ml (2h, RT). MBL-A was detected on the intima to media border as well as throughout the media (insert). Furthermore, extensive MBL-A deposition was seen at sites of necrosis (upper right corner).

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Key Properties

• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Immunogen: Purified mouse MBL-A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
• Buffer/Preservatives: 0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mannose binding protein A, MBP-A, Ra-reactive factor polysaccharide-binding component p28B, RaRF p28B, MBL-1

Quick Database Links

UniProt

P39039