MBD3L3 Antibody (C-term)

• Catalog Number: orb1937722
• Category: Antibodies
• Description: MBD3L3 Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: A6NE82
• MW: 23095 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Note: For research use only

Anti-MBD3L3 Antibody (C-term) at 1:1000 dilution + MCF-7 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of MBD3L3 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

MBD3L3 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MBD3L3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBD3L3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.