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Item 1 of 4
MBD3L3 Antibody (C-term)
Catalog Number: orb1937722
| Catalog Number | orb1937722 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB29958 |
| Tested applications | FC, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 23095 Da |
| Target | This MBD3L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the C-terminal region of human MBD3L3. |
| UniProt ID | A6NE82 |
| NCBI | NP_001157897.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Putative methyl-CpG-binding domain protein 3-like Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-MBD3L3 Antibody (C-term) at 1:1000 dilution + MCF-7 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of MBD3L3 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
MBD3L3 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MBD3L3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBD3L3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
