Recombinant Melan-A Antibody / Rabbit Monoclonal

SKU: orb2642109

Description

MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The optimal dilution of the recombinant Melan-A antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: MLANA/1761R
• Immunogen: Human protein was used as the immunogen for this recombinant Melan-A antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing FFPE human melanom with recombinant Melan-A antibody (clone MLANA/1761R). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

IHC testing of FFPE human skin with recombinant Melan-A antibody (clone MLANA/1761R). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Melan-A antibody (clone MLANA/1761R) as confirmation of integrity and purity.

Western blot testing of human COLO-38 cell lysate with recombinant Melan-A antibody (clone MLANA/1761R). Expected molecular weight ~20 kDa with possible doublet.

Quick Database Links

UniProt

Q16655