MART-1 Antibody / Melan-A

SKU: orb2640020

Description

MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: Full length recombinant human MART-1/Melan-A protein was used as the immunogen for this MART-1 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human melanoma stained with MART-1 antibody. HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

Western blot testing of human COLO-38 cell lysate with Melan-A antibody. Expected molecular weight ~20 kDa with possible doublet.

Quick Database Links

UniProt

Q16655