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Item 1 of 5
MARK2 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1499351
| Catalog Number | orb1499351 |
|---|---|
| Category | Antibodies |
| Description | MARK2 Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 1F10 |
| Tested applications | FC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant human MARK2 |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:400-800, IF=1:100-500, Flow-Cyt=1:100 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 88 kDa |
| Target | MARK2 |
| UniProt ID | Q7KZI7 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | ELKL motif kinase 1; ELKL motif kinase; EMK-1; EMK Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-MARK2 antibody, Dilution: 1:100, Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1:1000. Protocol, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (human appendix), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MARK2) Monoclonal Antibody, Unconjugated (orb1499351) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (Ph6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GEF H1) Monoclonal Antibody, Unconjugated (orb1499351) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (Ph6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GEF H1) Monoclonal Antibody, Unconjugated (orb1499351) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: MCF-7 cell lysate, Lane 2: SK-Br-3 cell lysate, Primary: Anti-MARK2 (orb1499351) at 1:2000 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 88 kD, Observed band size: 77/88 kD.
