Marein

SKU: orb1217909

Description

Marein shows neuroprotective effect on PC12 cell damage induced by methylglyoxal, which is due to a reduction of damage to mitochondria function and activation of the AMPK signal pathway, it may be a potent compound for preventing/counteracting diabetic encephalopathy.

Key Properties

• CAS Number: 535-96-6
• MW: 450.4
• Purity: >98% (HPLC)
• Formula: C₂₁H₂₂O₁₁
• SMILES: C1=CC(=C(C=C1/C=C/C(=O)C2=C(C(=C(C=C2)O[C@H]3[C@@H]([C@H]([C@@H]([C@H](O3)CO)O)O)O)O)O)O)O
• Target: Akt
• Solubility: In Vitro: DMSO : 25 mg/mL (55.51 mM)

Bioactivity

• In Vitro:
  Marein (0-1000 μM; 24 h) inhibits HDAC activity and TNFα-induced NF-κB activation with IC50 values of 100 and >200 μM, respectively. Marein (1.25-40 μM; 24 h) promotes glucose uptake in HepG2 cells. Marein (5-10 μM; 24 h) promotes GLUT1 translocation from intercellular vesicles to the plasma membrane, increases hepatic glycogen content and down-regulates expression levels of G6Pase and PEPCK in HepG2 cells. Marein (5-10 μM; 24 h) stimulates 2-NBDG uptake, and it can be reduced by STO-609 and compound C which is a inhibitor of AMPK. Marein (0-40 μM; 24 h) affects the cytotoxicity of MG in PC12 cells. Western blot analysis. Cell line: HepG2 cell line. Concentration: 5 and 10 μM. Incubation time: 24 hours. Result: Increased GLUT1 translocation to plasma membrane and decreased the phosphorylation level of AS160 and GSK-3β in HepG2 cells. Western blot analysis. Cell line: HepG2 cell line. Concentration: 10 μM. Incubation time: 0-10 hours. Result: Time- and dose-dependently induced phosphorylation of AMPK and Akt in HepG2 cells. Cell Viability Assay Cell line: PC12 cell line. Concentration: 0-40 μM. Incubation time: 24 hours. Result: Prevened MG-induced loss of viability in PC12 cells. Apoptosis Analysis Cell line: PC12 cell line. Concentration: 5-10 μM. Incubation time: 30 min. Result: Protected PC12 cells from MG-induced apoptosis. Western blot analysis. Cell line: PC12 cell line. Concentration: 5-10 μM. Incubation time: 30 min. Result: Increased phospho-AMPKα (Thr172) in PC12 cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Akt