MARCKS (phospho Ser159) rabbit pAb

• Catalog Number: orb769024
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MARCKS (phospho-Ser158)
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human MARCKS around the phosphorylation site of Ser158. AA range:126-175
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 35
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-MARCKS antibody, anti-MACS antibody, anti-PRK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MARCKS (Phospho-Ser158) Antibody.

Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using MARCKS (Phospho-Ser158) Antibody. The lane on the right is blocked with the phospho peptide.