MARCH10 Antibody

• Catalog Number: orb1246457
• Category: Antibodies
• Description: MARCH10 Antibody
• Target: 40238
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-SRNYPQPRTEE
• UniProt ID: Q8NA82
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MARCH10, membrane-associated ring finger (C3HC4) 1
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_001094345.1, NP_689811.2

orb1246457 (0.3 ug/ml) staining of Rat Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246457 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.