40238 Antibody

SKU: orb1246457

Description

40238 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SRNYPQPRTEE
• Target: 40238
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MARCH10, membrane-associated ring finger (C3HC4) 10 , FLJ35757, MARCH-X, RNF190, ring finger protein 190

orb1246457 (0.3 ug/ml) staining of Rat Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246457 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

40238

UniProt

Q8NA82

RefSeq (Protein):NP_001094345.1, NP_689811.2