MAPE Antibody (C-term)

• Catalog Number: orb1929852
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P78395
• MW: 57890 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:2000, IHC-P: 1:50~100, FC: 1:25
• Clone Number: RB18763
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Melanoma antigen preferentially expressed in tumor
• Note: For research use only
• NCBI: NP_996839.1, NP_006106.1, NP_996836.1, NP_996838.1, NP_996837.1

Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

MAPE Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-MAPE Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: 293 whole cell lysate. Lane 4: human testis lysate. Lane 5: human brain lysate. Lane 6: human kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.