MAP2K4 Antibody

SKU: orb1274500

Description

MAP2K4 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, KO/KD Validated, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MAP2K4 (NP_003001.1).
• Target: MAP2K4
• Molecular Weight: Observed: 44kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MAP2K4, JNK activating kinase 1, JNK-activated kinase 1, JNK-activating kinase 1, MAPK/ERK kinase 4, MAPKK4, JNKK1, PRKMK4, SAPKK1, SEK1, MEK4, MKK4, SAPK/ERK kinase 1, SERK1, JNKK, MAP kinase kinase 4, MAPKK 4, MEK 4, SAPK kinase 1, SAPKK-1, SKK1

Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (orb1274500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and MAP2K4 knockout (KO) HeLa cells, using MAP2K4 antibody (orb1274500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Quick Database Links

Gene Symbol

MAP2K4

UniProt

P45985