MAP1LC3A antibody(N-term)

• Catalog Number: orb1925347
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB59955
• Tested applications: IHC-P, WB
• Predicted Reactivity: Zebrafish
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: WB: 1:500, WB: 1:2000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 14272 Da
• Target: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-40 amino acids from human.
• UniProt ID: Q9H492
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Microtubule-associated proteins 1A/1B light chain
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-MAP1LC3A antibody (N-term) at 1:2000 dilution. Lane 1: Human brain tissue lysate. Lane 2: Mouse brain tissue lysate. Lane 3: Rat brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 14 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-MAP1LC3A antibody (N-term) at 1:500 dilution. Lane 1: Human brain tissue lysate. Lane 2: U-251 MG whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: Mouse brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 14 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human heart tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.