MAN2A1 Antibody

• Catalog Number: orb1247289
• Category: Antibodies
• Description: MAN2A1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KISSDIKSQNR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 150kDa band observed in Human Placenta lysates (calculated MW of 131kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human dermal fibroblasts.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MAN2A1
• UniProt ID: Q16706
• NCBI: NP_002363.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MAN2A1, mannosidase, alpha, class 2A, member 1, GO
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 150kDa band observed in Human Placenta lysates (calculated MW of 131kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human dermal fibroblasts.
• Expiration Date: 12 months from date of receipt.

orb1247289 (0.3 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Man2A1 and probed with orb1247289 (mock transfection in first lane).