MAML1 Antibody

• Catalog Number: orb1247292
• Category: Antibodies
• Description: MAML1 Antibody
• Target: MAML1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: VLPTCPMAEFALPR
• UniProt ID: Q92585
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Skeletal Muscle lysates (calculated MW of 108kDa according to NP_055572.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MAML1, mastermind-like 1 (Drosophila), Mam1, Mam-1
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_055572.1

orb1247292 (0.1 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247292 (3.8 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.