Malondialdehyde Antibody: Biotin

• Catalog Number: orb396651
• Category: Antibodies
• Description: Mouse monoclonal antibody against Malondialdehyde conjugated to Biotin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6H6
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); ICC/IF (1:50); FACS (1:50); FCM (1:50); ELISA (1:1000)
• Conjugation: Biotin
• Target: Malondialdehyde
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Malondialdehyde antibody, MDA antibody, Malondiald
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-514 was sufficient for detection of Malondialdehyde in 2 µg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 μM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alexa Fluor 633 F-Actin stain. (E, F) Malondialdehyde Antibody. (G, H) Composite.

Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 μg). Lane 3: Malondialdehyde-BSA (2.0 μg). Lane 4: BSA (0.5 μg). Lane 5: BSA (2.0 μg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Flow Cytometry analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 μM H2O2 for 24 hours.