Magic Red Caspase 3 & 7 Assay Kit

SKU: orb1409950

Description

The Magic Red reagent fluoresces upon cleavage by active caspase 3 and caspase 7 enzymes, enabling real-time detection of activity. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or flow cytometry.

Research Area

Cell Biology

Images & Validation

• Application Notes:
  1. Prepare samples and controls. 2. Reconstitute Magic Red with 100 or 400 µL DMSO, 3. Dilute Magic Red 1:5 with diH2O, 4. Add diluted Magic Red at 1:15-1:30 to suspension cells, 5. Incubate while protected from light. 6. Watch color start to develop within 15 minutes of addition of Magic Red, 7. If desired, label with additional stains, such as Hoechst, DAPI, or an antibody. 8. Analyze with a fluorescent microscope, fluorescent plate reader, or flow cytometer. Magic Red has a maximum excitation at 592 nm and emission at 628 nm. Good fluorescence images can be obtained at 510-560 nm excitation and >610 nm emission. If working with adherent cells, please see the manual for additional protocols.

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Key Properties

• Sample Types: Cell culture, tissue

Storage & Handling

• Storage: 2-8°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only