Magic Red Caspase 3 & 7 Assay Kit

SKU: orb1409949

Description

The Magic Red reagent fluoresces upon cleavage by active caspase 3 and caspase 7 enzymes, enabling real-time detection of activity. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or flow cytometry.

Research Area

Cell Biology

Images & Validation

• Application Notes:
  1. Prepare samples and controls. 2. Reconstitute Magic Red by adding DMSO. 3. Dilute Magic Red 1:10 by adding diH2O. 4. Add 20 µL Magic Red to each sample (~ 500 µL aliquot of cultured cells). 5. Incubate while protected from light. 6. Watch color start to develop within 15 minutes. 7. If desired, label with additional stains, such as Hoechst, DAPI, or an antibody. 8. If desired, fix cells. 9. Analyze with a fluorescence microscope or fluorescence plate reader. Magic Red has an optimal excitation at 592 nm and emission at 628 nm. If working with adherent cells, please see the manual for additional protocols.

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Key Properties

• Sample Types: Cell culture, tissue

Storage & Handling

• Storage: 2-8°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only