MAG Antibody (Center)

• Catalog Number: orb1928248
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21439
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 69069 Da
• Target: This MAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-466 amino acids from the Central region of human MAG.
• UniProt ID: P20916
• NCBI: NP_001186145.1, NP_002352.1, NP_542167.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Myelin-associated glycoprotein, Siglec-4a, MAG, GM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

MAG Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-MAG Antibody (Center) at 1:2000 dilution + Rat brain whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-MAG Antibody (Center) at 1:2000 dilution + Rat brain whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-MAG Antibody (Center) at 1:2000 dilution + Human brain whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-MAG Antibody (Center) at 1:2000 dilution. Lane 1: Human brain whole tissue lysate. Lane 2: Human cerebellum whole tissue lysate. Lane 3: Mouse brain whole tissue lysate. Lane 4: Mouse cerebellum whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.