MAD1L1 Antibody

SKU: orb1261233

Description

MAD1L1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1L1 (NP_001013859.1).
• Target: MAD1L1
• Molecular Weight: Observed: 95kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, MAD1-like protein 1, Mitotic checkpoint MAD1 protein homolog, HsMAD1, hMAD1, Tax-binding protein 181, MAD1L1, MAD1, TXBP181

Western blot analysis of extracts of various cell lines, using MAD1L1 antibody (orb1261233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Immunohistochemistry of paraffin-embedded mouse liver using MAD1L1 antibody (orb1261233) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using MAD1L1 antibody (orb1261233) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using MAD1L1 antibody (orb1261233).

Quick Database Links

Gene Symbol

MAD1L1

UniProt

Q9Y6D9