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Catalog Number | orb1252394 |
---|---|
Category | Antibodies |
Description | Macrophage / Histiocytoma Marker Antibody [D11] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | D11 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | IgG1, kappa |
Immunogen | A membrane preparation from human hepatocytes was used as the immunogen for the Macrophage / Histiocytoma Marker antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Macrophage / Histiocytoma Marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
UniProt ID | NotKnown |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Note | For research use only |
Application notes | Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Macrophage / Histiocytoma Marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage antibody (D11).
Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage antibody (D11).
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