M Sema5a Antibody

SKU: orb1931539

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Neuroscience

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1006-1035 amino acids from mouse Sema5a. Antigen Region: 1006-1035 aa.
• Target: SEMA5A
• Molecular Weight: 120615 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF

Western blot analysis of mouse anti-Semaphorin 5A Pab in mouse brain tissue lysates (35 ug/lane). Semaphorin 5A (arrow) was detected using the purified Pab.

Anti-Semaphorin 5A (C-term) at 1:1000 dilution + U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 121 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

SEMA5A

UniProt

Q13591

RefSeq (Protein):NP_003957.2