M Sema5a Antibody

• Catalog Number: orb1931539
• Category: Antibodies
• Description: M Sema5a Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: Q13591
• MW: 120615 Da
• Tested applications: WB
• Dilution range: WB - 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: SEMAF
• Research Area: Neuroscience
• Note: For research use only

Western blot analysis of mouse anti-Semaphorin 5A Pab in mouse brain tissue lysates (35 ug/lane). Semaphorin 5A (arrow) was detected using the purified Pab.

Anti-Semaphorin 5A (C-term) at 1:1000 dilution + U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 121 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.