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M Sema5a Antibody
Catalog Number: orb1931539
| Catalog Number | orb1931539 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB8966 |
| Tested applications | WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 120615 Da |
| Target | This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1006-1035 amino acids from mouse Sema5a. |
| UniProt ID | Q13591 |
| NCBI | NP_003957.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of mouse anti-Semaphorin 5A Pab in mouse brain tissue lysates (35 ug/lane). Semaphorin 5A (arrow) was detected using the purified Pab.
Anti-Semaphorin 5A (C-term) at 1:1000 dilution + U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 121 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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